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Real Time Pcr Detection Test Kit Food Safety Listeria Monocytogenes

BIOPFS-0003
qPCR / Real Time PCR/ PCR
Biopremier
BIOPFS-0003: 100 reactions - FAM, ROX
BIOPFS-sp0003: 10 reactions - FAM, ROX
(pdf) Instructions for use

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Real Time Pcr Detection Test Kit Food Safety Listeria Monocytogenes

Detection of L. monocytogenes in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO 11290 (recommended). For use in real time instruments with FAM and ROX channels.

BACKGROUND: Listeria monocytogenes, the causative agent of listeriosis, is of clinical relevance for humans. L. monocytogenes infections have a high mortality rate ranking among the most frequent cause of human death due to foodborne illnesses. Listeriosis mainly affects specific population groups, with increased susceptibility: young, old, pregnant and immunodepressed. In particular, pregnant women are of main concern, as listeriosis causes spontaneous abortion or stillbirth. Normally Listeria is transferred to humans through raw milk, soft-ripened cheeses, raw vegetables, poultry, raw meats and raw or smoked fish. The ability of Listeria spp. to grow at low temperatures (3ºC) increases the risk of infection. Even though there are standard methods for the detection and enumeration of this pathogen, usually they are culture based and time consuming. Today, there are approved methods for PCR base detection of L. monocytogenes unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT L. monocytogenes uses real-time PCR for the detection of L. monocytogenes in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Listeria monocytogenes
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 100 reactions or 10 reactions

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