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Real Time Pcr Detection Test Kit Food Safety Listeria Monocytogenes
Item code
BIOPFS-0003
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Biopremier
Variants
BIOPFS-0003: 100 reactions - FAM, ROX
BIOPFS-sp0003: 10 reactions - FAM, ROX
Instructions
(pdf)
Instructions for use
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Detection of L. monocytogenes in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO 11290 (recommended). For use in real time instruments with FAM and ROX channels.
BACKGROUND:
Listeria monocytogenes, the causative agent of listeriosis, is of clinical relevance for humans. L. monocytogenes infections have a high mortality rate ranking among the most frequent cause of human death due to foodborne illnesses. Listeriosis mainly affects specific population groups, with increased susceptibility: young, old, pregnant and immunodepressed. In particular, pregnant women are of main concern, as listeriosis causes spontaneous abortion or stillbirth. Normally Listeria is transferred to humans through raw milk, soft-ripened cheeses, raw vegetables, poultry, raw meats and raw or smoked fish. The ability of Listeria spp. to grow at low temperatures (3ºC) increases the risk of infection. Even though there are standard methods for the detection and enumeration of this pathogen, usually they are culture based and time consuming. Today, there are approved methods for PCR base detection of L. monocytogenes unique DNA sequences, in particular using real time PCR and specific fluorescent probes.
PRODUCT DESCRIPTION:
REAL TIME DETECTION KIT L. monocytogenes uses real-time PCR for the detection of L. monocytogenes in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).
PRODUCT FEATURES:
-Amplification and detection of Listeria monocytogenes
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 100 reactions or 10 reactions
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
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qPCR / Real Time PCR/ PCR
Others
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MFIA
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