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REAL TIME PCR DETECTION TEST KIT FOOD SAFETY Campylobacter jejuni

BIOPFS-0005
qPCR / Real Time PCR/ PCR
Biopremier
BIOPFS-0005: 100 reactions - FAM, ROX
BIOPFS-sp0005: 10 reactions - FAM, ROX
(pdf) Instructions for use

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REAL TIME PCR DETECTION TEST KIT FOOD SAFETY Campylobacter jejuni

Detection of Campylobacter jejuni in food products, animal feedstuff and environmental samples. Pre-enrichment broth according ISO 10272 - 1:2006 (recommended). The kit is suitable for use in real time instruments with FAM and ROX detection channels.

BACKGROUND: The incidence of Campylobacter-associated food poisoning has gradually increased, and the organism is now considered a leading cause of bacterial gastroenteritis worldwide. These infections can also cause extraintestinal diseases and severe long-term complications. These bacteria are commensal in poultry, cattle, pigs, and other animals. As a consequence, food from animal origin may become contaminated. Thus, infections mainly occur by consumption of contaminated water, raw or inadequately pasteurized milk, and undercooked meat. Currently, Campylobacter jejuni accounts for about 80% of Campylobacter infections in humans. Campylobacter spp. are bacteria with special culture requirements, including a microaerobic environment. Long exposure to the normal atmosphere during sample processing, together with the selective media usually used for isolation, may not favor growth, preventing correct detection. Furthermore, culture-based methods are time-consuming and alternative methods are needed in order to quickly detect and identify C. jejuni in food.
In vitro amplification of DNA by the polymerase chain reaction (PCR) has become a powerful alternative in microbiological diagnostics due to its rapidity and accuracy. Today there are available methods for PCR based detection of C. jejuni unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT Campylobacter jejuni uses real-time PCR for the detection of Campylobacter jejuni in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Campylobacter jejuni
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions

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