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REAL TIME PCR DETECTION TEST KIT beverages Zygosaccharomyces bailii

BIOPFS-0020
qPCR / Real Time PCR/ PCR
Biopremier
BIOPFS-0020: 100 reactions - FAM, ROX
BIOPFS-sp0020: 10 reactions - FAM, ROX
(pdf) Instructions for use

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REAL TIME PCR DETECTION TEST KIT beverages Zygosaccharomyces bailii

Detection Z. bailii in wine and other beverages. The kit is suitable for use in real time instruments with FAM and ROX detection channels.

BACKGROUND: Zygosaccharomyces bailii is a food spoilage yeast with high importance for wine, beverage, and food industry, because of its ability to grow in the presence of weak acid preservatives such as benzoic and sorbic acids. It has been identified as a cause of spoilage in alcoholic beverages such as wine and in foods such as mustard and soft drinks. Z. bailii is responsible for significant economic loss in the wine and food industry and its efficient detection is of most importance. This detection is mostly performed using classical microbiological methods that rely on the growth of Z. bailii on selective media. This media is often exploiting its ability to grow in the presence of sorbate or other weak acids. The growth of Z. bailii on this media typically occurs between 4 and 5 days. Therefore, methods to shorten the time needed for the detection of Z. bailii, in wine, beverages, and food are desirable. Thus, in vitro amplification of DNA by polymerase chain reaction (PCR), in particular, using real time PCR and specific fluorescent probes, is a powerful and reliable alternative for Z. bailii detection, providing results in much shorter times and allowing more efficient control procedures.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT Zygosaccharomyces bailii uses real-time PCR for the detection of Zygosaccharomyces bailii in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target microrganism as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Zygosaccharomyces bailii
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Target: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions

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