Real Time PCR Detection Kit Xylella fastidiosa

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Xylella fastidiosa

Background:Xylella fastidiosa detection by using qPCR.
Xylella fastidiosa is an aerobic, Gram-negative bacterium belonging to the Xanthomonadaceae family. It is a plant pathogen transmitted exclusively by xylem fluid-feeding insects. Many plant diseases are due to symptomatic infections of X. fastidiosa, including bacterial leaf scorch, oleander leaf scorch, coffee leaf scorch, alfalfa dwarf, phony peach disease, and the economically important ones: Pierce’s disease of grapes and citrus variegated chlorosis. In Europe it has attacked olive trees causing the olive quick decline syndrome. X. fastidiosa can be divided into four subspecies that affect different plants and have separate origins. X. fastidiosa subsp. fastidiosa is the most studied, but X. fastidiosa subsp. multiplex, X. fastidiosa subsp. pauca and X. fastidiosa subsp. sandyi are also relevant. There is significant variation in symptoms between diseases, though some common symptoms are expressed across affected species. On a macroscopic scale, plants infected with a X. fastidiosa-related disease exhibit symptoms of water deficiencies, manifesting as leaf scorching, stunting in leaves, fruit, and overall plant height. The bacterium has a two-part life cycle: inside an insect vector, and inside a susceptible plant and can be found worldwide.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control