Real Time PCR Detection Kit Verticillium dahliae

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Verticillium dahliae

Background:Verticillium dahliae detection by using qPCR.
Verticillium dahlia is a fungal plant pathogen with a cosmopolitan distribution that can infect many plant genera. It is one of the responsible for the Verticillium wilt and over 200 dicotyledonous species have been identified as susceptible hosts of this pathogen. Agricultural hosts include spinach, strawberries, lettuce, and artichokes, among others. Symptoms are not commonly used to identify this pathogen because of their non-specific nature and also may vary depending on the specific host that the pathogen is infecting. The most common way to introduce V. dahliae in the field is by infected stock being planted. Once the pathogen is in the field it can be moved by wind, water, and soil. This a very difficult pathogen to control because it can persist in the soil for years without a susceptible host. V. dahliae can cause significant losses in yield due to reduced photosynthesis if the plant survives.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control