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Real Time PCR Detection Kit Shiga toxin 2 Escherichia coli
Item code
GPCRsStx2
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GPCRs
Variants
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX
970
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Background:
Shiga toxin 2 Escherichia coli detection by using qPCR.
Shiga toxin is a family of related toxins with two major groups, Stx1 and Stx2. The most common sources of Shiga toxin are the bacteria Shigella dysenteriae and the Shiga toxigenic group of Escherichia coli (STEC), which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). Shiga toxins have many names, which can be used interchangeably (shiga toxins, shiga-like toxins, cytotoxins, verocytotoxins, verotoxins). STEC lives in the guts of ruminant animals, including cattle, goats, sheep, deer, and elk. The major source of human illnesses is cattle, where STEC generally does not make animals become sick. The symptoms of STEC infections often include severe stomach cramps, diarrhea (often bloody), and vomiting. Most patients recover within 10 days, but in young children and the elderly, the infection may lead to a life-threatening disease, such as hemolytic uraemic syndrome. Haemolytic uraemic syndrome is characterized by acute renal failure, hemolytic anemia, and thrombocytopenia. Stx-2 has been found to be about 400 times more toxic than Stx-1.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
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Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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