This real-time PCR (qPCR) kit allows detecting Salmonella Dublin, Typhimurium and Salmonella spp
REAL TIME PCR DETECTION KIT Salmonella Dublin, Typhimurium and Salmonella spp Bovichek triplex contains VIC, FAM and ABY labeled probes for the specific identification and quantification of Salmonella spp, S. Dublin, and S. Typhimurium respectively.
This kit provides every component of the qPCR reaction (buffers, enzyme, dNTPs, specific primers and probes for the pathogens.
A synthetic Salmonella spp, S. Dublin, and S. Typhimurium positive control is provided for relative and absolute quantification.
SUMMARY AND EXPLANATION:
Salmonella enterica subspecies enterica is a major pathogen in various animal species including cattle. In this species, the two most important serovars are Typhimurium and Dublin. Serovar Typhimurium is mainly causing enteritis. By contrast serovar Dublin is particularly adapted to this species (host-adapted) and, in addition to enteritis, it may also cause severe systemic infections such as septicemia, pneumonia, polyarthritis, or abortion. Sensitive and specific detection of Salmonella serovars from biological or environmental samples is essential to implement efficient control measures adapted to the serovar involved. Combining selective enrichment with real time PCR greatly improves the sensitivity of Salmonella spp detection, especially S. Dublin, while providing next-day results.
This is a triplex real-time PCR which allows rapid, sensitive, and specific detection of Salmonella spp, S. Dublin, and S. Typhimurium, especially after selective enrichment.
- Test per kit: 50 samples
- qPCR Results within two hours