Real Time PCR Detection Kit Rickettsia spp.

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Rickettsia spp.

Background:Detection of Rickettsia spp. by using qPCR.
Rickettsia is a genus of non-motile, Gram-negative, non-spore-forming, highly pleomorphic bacteria. The name rickettsia is often used for any member of the Rickettsiales. They are obligate intracellular parasites: Rickettsia survival depends on entry, growth, and replication within the cytoplasm of eukaryotic host cells (typically endothelial cells). As a consequence of this, Rickettsia cannot live in artificial nutrient environments but can grow either in tissue or embryo cultures. In the past, they were positioned somewhere between viruses and true bacteria. However, Rickettsial organisms possess true cell walls similar to other gram-negative bacteria. Rickettsia species are carried by many ticks, fleas, and lice, and cause diseases in humans such as typhus, rickettsialpox, Boutonneuse fever, African tick bite fever, Rocky Mountain spotted fever, Flinders Island spotted fever and Queensland tick typhus (Australian Tick Typhus). They have also been associated with a range of plant diseases. Despite the similar name, Rickettsia bacteria do not cause rickets, which is a result of vitamin D deficiency.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control