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Real Time PCR Detection Kit Neonatal Pig Diarrhoea GeneExpres Panel (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens)
Item code
GPCRsDiarrhoeaPanel6
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GPCRs
Variants
6 targets: 6 x 100 reactions - FAM
6 targets + IC: 6 x 100 reactions - FAM, HEX
Monodose 6 targets: 6 x 96 qPCR tubes - FAM
Monodose 6 targets + IC: 6 x 96 qPCR tubes - FAM, HEX
931
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Background:
The expression of six virulence factors (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens) by using qPCR.
Of all the diseases in the sucking piglet, diarrhea is the most common and probably the most important. The consequences of profuse, watery diarrhea will be a rapid loss of water, electrolytes, and nutrients. Due to the limited body reserves of the newborn piglet, this will soon lead to a severe condition and the piglet may die within hours.
The Kit allows the evaluation mRNA expression of six virulence factors from different organisms related to neonatal pig diarrhea. The E. coli genes: toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine and faeG, coding for the F4 fimbriae. The gene tcdA coding for the C. difficile toxin A. Genes cpa and cpb2 coding for the alpha and beta 2 toxins from C. perfringens.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
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Equipments ELISA
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Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
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Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Others
Lateral Flow Test
MFIA
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