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Real Time PCR Detection Kit Neonatal Pig Diarrhoea GeneExpres Panel (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens)

GPCRsDiarrhoeaPanel6
qPCR / Real Time PCR/ PCR
GPCRs
6 targets: 6 x 100 reactions - FAM
6 targets + IC: 6 x 100 reactions - FAM, HEX
Monodose 6 targets: 6 x 96 qPCR tubes - FAM
Monodose 6 targets + IC: 6 x 96 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Neonatal Pig Diarrhoea GeneExpres Panel (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens)

Background:The expression of six virulence factors (faeG, eae, toxA from Escherichia coli, tcdA from Clostridium difficile and cpa, cpb2 from Clostridium perfringens) by using qPCR.
Of all the diseases in the sucking piglet, diarrhea is the most common and probably the most important. The consequences of profuse, watery diarrhea will be a rapid loss of water, electrolytes, and nutrients. Due to the limited body reserves of the newborn piglet, this will soon lead to a severe condition and the piglet may die within hours.
The Kit allows the evaluation mRNA expression of six virulence factors from different organisms related to neonatal pig diarrhea. The E. coli genes: toxA, coding for the heat-labile enterotoxin (LT); eae, coding for the intimine and faeG, coding for the F4 fimbriae. The gene tcdA coding for the C. difficile toxin A. Genes cpa and cpb2 coding for the alpha and beta 2 toxins from C. perfringens.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control

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