Real Time PCR Detection Kit Mycobacterium avium subsp. paratuberculosis

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Mycobacterium avium subsp. paratuberculosis

Background:Detection of Mycobacterium avium subspecies paratuberculosis (MAP) by using qPCR.
Mycobacterium avium subspecies paratuberculosis (MAP) is an obligate pathogenic bacterium in the genus Mycobacterium, often abbreviated M. paratuberculosis. It is the causative agent of Johne’s disease, which affects ruminants such as cattle, and is also perhaps responsible for Crohn’s disease in humans. Recent studies have shown that MAP present in milk can survive pasteurization, which has raised human health concerns. It is heat resistant and is capable of sequestering itself inside white blood cells, which may contribute to its persistence in milk. It has also been reported to survive chlorination in municipal water supplies. MAP is a slow-growing organism and is difficult to culture. Detection is very limited in fresh tissues, food, and water.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control