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Real Time PCR Detection Kit Methicillin resistant Staphylococcus aureus - TwoTarget

GPCRsMRSA2T
qPCR / Real Time PCR/ PCR
GPCRs
2 targets: 2 x 100 reactions - FAM
2 targets + IC: 2 x 100 reactions - FAM, HEX

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Real Time PCR Detection Kit Methicillin resistant Staphylococcus aureus - TwoTarget

Background:Designed for qPCR detection of Methicillin-resistant strains of the species Staphylococcus aureus (MRSA). Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for several difficult-to-treat infections in humans. It is also called multidrug-resistant Staphylococcus aureus and oxacillin-resistant Staphylococcus aureus (ORSA). MRSA is any strain of Staphylococcus aureus that has developed resistance to β-lactam antibiotics, which include the penicillins (methicillin, dicloxacillin, nafcillin, oxacillin, etc.) and the cephalosporins.
The evolution of such resistance does not cause the organism to be more intrinsically virulent than strains of Staphylococcus aureus that have no antibiotic resistance, but resistance does make MRSA infection more difficult to treat with standard types of antibiotics and thus more dangerous. Methicillin resistance arises by the acquisition of a staphylococcal cassette chromosome SCCmec and is conferred by the Methicillin resistance gene (MRG). Expression of this gene yields PBP2a, a penicillin-binding protein with reduced affinity for β-lactam rings (the primary active site of the β-lactam antibiotics). Earlier studies have underlined the important role of the enzymatic family FemXAB (factor essential for methicillin resistance) in the formation of the pentaglycine bridges that stabilize peptidoglycan chains inS. aureus. The MRG test detects the gene responsible for antibiotic resistance. StaAur test detects a chromosomal gene-specific of S. aureus.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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