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Real Time PCR Detection Kit Methicillin resistant Staphylococcus aureus - TwoTarget
Item code
GPCRsMRSA2T
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GPCRs
Variants
2 targets: 2 x 100 reactions - FAM
2 targets + IC: 2 x 100 reactions - FAM, HEX
893
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Background:
Designed for qPCR detection of Methicillin-resistant strains of the species Staphylococcus aureus (MRSA). Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for several difficult-to-treat infections in humans. It is also called multidrug-resistant Staphylococcus aureus and oxacillin-resistant Staphylococcus aureus (ORSA). MRSA is any strain of Staphylococcus aureus that has developed resistance to β-lactam antibiotics, which include the penicillins (methicillin, dicloxacillin, nafcillin, oxacillin, etc.) and the cephalosporins.
The evolution of such resistance does not cause the organism to be more intrinsically virulent than strains of Staphylococcus aureus that have no antibiotic resistance, but resistance does make MRSA infection more difficult to treat with standard types of antibiotics and thus more dangerous. Methicillin resistance arises by the acquisition of a staphylococcal cassette chromosome SCCmec and is conferred by the Methicillin resistance gene (MRG). Expression of this gene yields PBP2a, a penicillin-binding protein with reduced affinity for β-lactam rings (the primary active site of the β-lactam antibiotics). Earlier studies have underlined the important role of the enzymatic family FemXAB (factor essential for methicillin resistance) in the formation of the pentaglycine bridges that stabilize peptidoglycan chains inS. aureus. The MRG test detects the gene responsible for antibiotic resistance. StaAur test detects a chromosomal gene-specific of S. aureus.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
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Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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ELISA
qPCR / Real Time PCR/ PCR
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Lateral Flow Test
MFIA
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