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Real Time PCR Detection Kit Gluten
Item code
GPCRsGluten
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GPCRs
Variants
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX
849
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Background:
Gluten detection by using qPCR.
Celiac disease is an autoimmune disorder associated with an intolerance to gluten proteins mainly present in wheat, barley, and rye. It is characterized by an inflammatory response that leads to atrophy of the small intestine and malabsorption. Celiac disease is estimated to affect 1 % of people worldwide. The treatment of gluten-related disorders is based on a lifelong gluten-free diet. Accordingly, reliable and sensitive methods are required to detect the presence of gluten contamination in food samples. Traditional techniques rely on the detection of the proteins based on specific antibodies, but recent approaches focus on detecting the DNA of cereals with gluten content. This assay does not detect the avenin from oat (Avena sativa) which despite being a different protein could sometimes, but to a much lesser extent, produce a reaction in people with celiac disease.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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