Background:Detection of Foodborne Poisoning (Salmonella spp., Listeria monocytogenes and shiga toxins 1 and 2) by using qPCR.
Foodborne poisoning is any illness resulting from food contaminated with a disease-causing agent such as pathogenic bacteria, viruses, or parasites. In addition, some poisonous chemicals, or other harmful substances can also cause foodborne diseases if they are present in food. Symptoms often include vomiting, fever, and aches, and may include diarrhea. The foodborne illness usually arises from improper handling, preparation, or food storage. Good hygiene practices before, during, and after food preparation can reduce the chances of contracting an illness. The action of monitoring food, to ensure that it will not cause foodborne illness, is known as food safety. As bacteria are a common cause of foodborne illness, there are three main targets controlled by food safety normative: Salmonella spp., Listeria monocytogenes, and more recently the Shiga toxigenic group of Escherichia coli (STEC).
Salmonella is closely related to the Escherichia genus and are found worldwide in cold and warm-blooded animals (including humans), and in the environment. Salmonella species are facultative intracellular pathogens that enter cells via macropinosomes. Listeria monocytogenes is the bacterium that causes the infection listeriosis. It can grow and reproduce inside the host’s cells and is one of the most virulent food-borne pathogens, with 20 to 30 percent of clinical infections resulting in death. Shiga toxin is a family of related toxins with two major groups, Stx1 and Stx2. The most common Shiga toxin sources are the Shigella dysenteriae and the Shiga toxigenic group of Escherichia coli (STEC), which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). Shiga toxins have many names, which can be used interchangeably (shiga toxins, shiga-like toxins, cytotoxins, verocytotoxins, verotoxins). STEC live in the guts of ruminant animals, including cattle, goats, sheep, deer, and elk.
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control