Real Time PCR Detection Kit Dobrava-Belgrade virus

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Dobrava-Belgrade virus

Background:Detection of the “phylogenetic core” of the Dobrova-Belgrade virus by using qPCR.
Due to the vast genetic diversity described to date under this name, the specificity (inclusivity) of this qPCR test would cover the core of DOBV. This cluster includes the reference strains described for the virus and the Dobrava-Belgrade virus. Sequences of the out-groups may need some review and taxonomic rearrangements. In fact, Klempa et al. (Arch Virol, 2012) propose a subdivision of the species Dobrava-Belgrade virus into four related genotypes that show characteristic differences in their phylogeny, specific host reservoirs, geographical distribution, and pathogenicity for humans.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control