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Real Time PCR Detection Kit Cell-free human DNA (cfDNA)
Item code
GPCRscfhDNA
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GPCRs
Variants
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX
781
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Background:
Cell-free human DNA detection by using qPCR.
The target is a multiple-copy gene, 200 copies per genome, with a slow evolutionary rate. Due to the multiple-copy and the high degree of conservation, can be used for quantification of low amounts of circulating cell-free DNA (cfDNA) present in human blood. Cell-free DNA is becoming an important clinical analyte for prenatal testing, cancer diagnosis, and cancer monitoring. A multiple‑copy gene provides an increase in signal amplification, allowing the detection of low DNA amounts.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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