Real Time PCR Detection Kit Brachyspira pilosicoli

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Brachyspira pilosicoli

Background:Brachyspira pilosicoli detection by using qPCR.
Brachyspira pilosicoli (formerly Serpulina pilosicoli) is a Gram-negative, spiral-shaped, obligate anaerobe bacterium member of the Spirochaete family. It causes intestinal spirochaetosis in pigs, but it has also been isolated from dogs, birds and mice and also can cause zoonotic infection in humans. Dogs are thought to be a source of infection for people. Infection of B. pilosicoli is acquired through the fecal-oral route. Once in the alimentary tract, the bacterium produces edema, hemorrhage, and infiltration by inflammatory cells. The consequences are the sloughing of cells into the intestinal lumen, malabsorption, and secretory diarrhea. Affected pigs may be inappetent and grow slowly.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control