Real Time PCR Detection Kit Bovine viral diarrhea virus-Border disease virus

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Bovine viral diarrhea virus-Border disease virus

Background:Bovine viral diarrhea virus Border disease virus detection by using qPCR.
Bovine viral diarrhea viruses are enveloped, single-stranded, positive-sense RNA viruses members of the Pestivirus genus and belonging to the family Flaviviridae. It is considered one of the most significant infectious diseases in the livestock industry worldwide due to its high prevalence, persistence, and clinical consequences. The most common consequences of Bovine viral diarrhea virus infection are respiratory and reproductive problems. In addition to the transient infection, when the virus infects a susceptible cow during a certain period of gestation can result in the production of a persistently infected fetus; capable of excreting large amounts of the virus throughout its life. Transient infections include diarrhea, calf pneumonia, decreased milk production, reproductive disorders, increased occurrence of other diseases, and death. Both types, transient and persistently infected animals, can spread infectious viruses and transmission occurs both horizontally and vertically. The virus is transmitted via direct contact, bodily secretions, and contaminated fomites. The assay may detect the three described BVDV genotypes but, due to the observed genetic variability of this species, emerging genotypes and subtypes may differ from the ones used for the present design. Border disease virus, also detected, can cause a significant problem in flocks of sheep, causing an abortion in affected animals or the birth of weak lambs.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control