Real Time PCR Detection Kit Bovine herpesvirus 1

qPCR / Real Time PCR/ PCR
Target: 100 reactions - FAM
Target + IC: 100 reactions - FAM, HEX
Monodose Target: 24 qPCR tubes - FAM
Monodose Target + IC: 24 qPCR tubes - FAM, HEX

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Real Time PCR Detection Kit Bovine herpesvirus 1

Background:Detection of Bovine herpesvirus 1 (BoHV-1) virus by using qPCR.
Bovine herpesvirus 1(BoHV-1) is a dsDNA virus of the family Herpesviridae, known to cause several diseases worldwide in cattle including rhinotracheitis, vaginitis, balanoposthitis, abortion, conjunctivitis and enteritis. BoHV-1 is also a contributing factor in shipping fever, also known as Bovine Respiratory Disease (BRD). Infection may occur in cattle of any age but is most commonly seen between the ages of 6 to 18 months. It is spread horizontally through sexual contact, artificial insemination, and aerosol transmission and it may also be transmitted vertically across the placenta. BoHV-1 can cause both clinical and subclinical infections, depending on the virulence of the strain. Like other herpesviruses, BoHV-1 causes a lifelong latent infection and sporadic shedding of the virus. The sciatic nerve and trigeminal nerve are the sites of latency. A reactivated latent carrier is normally the source of infection in a herd. The clinical signs displayed are dependent on the virulence of the strain. Some countries in Europe have successfully eradicated the disease by applying a strict culling policy.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control