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Real Time PCR Detection Kit Actinobacillus pleuropneumoniae Apx Toxin-type Panel (ApxI, ApxII and ApxIII)
Item code
GPCRsActPlePanel3
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GPCRs
Variants
3 targets: 3 x 100 reactions - FAM
3 targets + IC: 3 x 100 reactions - FAM, HEX
Monodose 3 targets: 3 x 96 qPCR tubes - FAM
Monodose 3 targets + IC: 3 x 96 qPCR tubes - FAM, HEX
700
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Background:
The panel comprises a series of species-specific targeted reagents designed for the detection of the three Apx toxins of the Actinobacillus pleuropneumoniae (ApxI, ApxII and ApxIII) by using qPCR.
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a non-motile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It is a respiratory pathogen found in pigs and was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. The main disease associated with this bacterium is porcine pleuropneumonia, a highly contagious respiratory disease, affecting primarily young pigs (usually less than 6 months). The symptoms include respiratory distress, bloodstained discharge (usually frothy) from the mouth, fever, anorexia, mild diarrhea, cyanosis, lethargy, and spontaneous abortion in sows. Mortality rates can reach 20-80 % in fattening pigs, with similarly high morbidity. Pigs that do survive the disease remain as carriers and spread the bacterium to other swine. A. pleuropneumoniae has a profound economic impact on pork production and pig farmers. Two biotypes and at least 15 serotypes are present. Several virulence factors account for the remarkable pathogenicity of A. pleuropneumoniae. One of the more important is the production and release of the Apx toxins, which are subdivided into four types: ApxI through ApxIV (ApxIV is expressed in all serotypes). Each serotype is described to express different amounts of the four Apx toxins.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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