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REAL TIME DETECTION KIT P-35S, T-NOS and P-FMV

BIOPFS-0017
qPCR / Real Time PCR/ PCR
Biopremier
50/50/50 or 5/5/5 reactions

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BACKGROUND: Genetically modified food and feed products have become a reality. Since 1994, the date of their initial commercialization, about 150 genetically modified plants have received approval for use as food or feed. In most countries, such use is highly regulated with the implementation of compliance measures. The enforcement of this legislation is essential for the efficient detection of genetically modified organisms (GMOs) in food and feed products. Considering their large diversity (different Genetically modified (GM) elements in various species), the application of a generic screening for the presence of GM material is most often the first step in GMO analysis. Such qualitative screening methods provide a presence/absence response and help to reduce the number of subsequent identification analyses. The promoter 35S obtained from cauliflower mosaic virus (CaMV) and the terminator NOS from Agrobacterium tumefaciens are the most frequent elements present in transgenic material in food. However, these regulatory elements do not cover such important transgenic events as MON89788 soy, H7-1 sugar beet, or GT73 rape. Therefore, in order to ensure the widest screening, it is important to also detect a promotor from Figwort Mosaic Virus (P-FMV) and real-time PCR methods, using specific fluorescent probes, targeting these elements have shown to be a reliable strategy to perform this screening. PRODUCT DESCRIPTION: REAL TIME DETECTION KIT P-35S, T-NOS and P-FMV uses real-time PCR for the detection of the genetically modified elements P-35S, T-NOS and P-FMV in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the genetically modified elements as well as a positive control and an internal control (IC). PRODUCT FEATURES: -Amplification and detection of P-35S, T-NOS and P-FMV -Real-time PCR with rapid hot-start Taq DNA polymerase -Internal Control to exclude false-negative results -PCR- platforms: runs on all established standard real-time PCR- platforms -Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously -Channel Target: FAM, Channel IC: ROX -Available formats: Kits with 50 reactions for each target or 5 reactions for each target

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