Enzyme immunoassay for the quantitative determination of Peanut in food.
Background: Peanut (Arachis hypogaea) belongs to the legumes. With 25 % the fraction of proteins in peanuts is very high. Many of these proteins are known for being allergenic, such as Arachins and Conarachins which are contained in relative high amounts. For this reason peanut represents one of the most important food allergens. For peanut allergic persons hidden peanut allergens in food are a critical problem. Already very low amounts of peanuts can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, peanut allergic persons must strictly avoid the consumption of peanuts or peanut containing food. Cross-contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of peanut residues in foods cannot be excluded. For this reason sensitive detection systems for peanut residues in foodstuffs are required.
Peanut ELISA represents a highly sensitive detection system and is particularly capable of the quantification of peanut residues in cookies, cereals, ice cream, chocolate and hazelnut.
Description: The Peanut quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against peanut proteins is bound on the surface of a microtiter plate. Peanut containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against peanut proteins is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of peanut is directly proportional to the colour intensity of the test sample.
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Peanut Standards: 0, 1, 4, 10, 40 ppm;
- ELISA reader at 450 nm;
- Standard range: 1 - 40 ppm;
- Sensitivity analytical: 0.2 ppm.