ParoReal Kit Trichinella spiralis & britovi

Detection and differentiation of the internal transcribed spacer of Trichinella spiralis and Trichinella britovi

Background: Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide, four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.

Description: ParoReal Kit Trichinella spiralis & britovi uses real-time PCR for the detection of the internal transcribed spacer of Trichinella spiralis and T. britovi. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: internal transcribed spacer of Trichinella spiralis & T. britovi
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5