Enzyme Immunoassay for the quantitative determination of Mollusc Tropomyosin in food.
Background: Not only because of their cross-reactivity to house dust mites and crustaceans, molluscs represent an important group of food allergens. In this regard tropomyosin, which can be found in all common mollusc species, is the most important protein. In cooked and uncooked mollusc extracts this protein partly represents a high amount of total protein.
For mollusc allergic persons hidden mollusc proteins in food are a critical problem. Already very low amounts of the allergen can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, mollusc allergic persons must strictly avoid the consumption of mollusc containing food. Cross-contamination, mostly in consequence of the production process, is often noticed. This explains why in many cases the existence of mollusc residues in food cannot be excluded. For this reason sensitive detection systems for mollusc residues in foodstuffs are required.
The Molluscs (Tropomyosin) ELISA represents a highly sensitive detection system for tropomyosin (from Helix aspersa) and is particularly capable of the quantification of mollusc residues in fish products, soups, dressings, bakery products and meat products.
Description: The Molluscs (Tropomyosin) quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against mollusc tropomyosin is bound on the surface of a microtiter plate. Tropomyosin containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against tropomyosin is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of tropomyosin is directly proportional to the colour intensity of the test sample.
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Tropomyosin Standards: 0, 10, 40, 100, 400 ppb;
- ELISA reader at 450 nm;
- Standard range: 10 - 400 ppb;
- Sensitivity analytical: 1.7 ppb.