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Microbial DNA Extraction Kit - Bead beating Biopremier
Item code
BIOPEXT-0619.50
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Biopremier
Variants
BIOPEXT-0619.50: 50 preps
Instructions
(pdf)
Instructions for use
1060
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The Biopremier Microbial DNA extraction Kit – Bead beating is designed for a fast and efficient purification of microbial DNA
PRODUCT DESCRIPTION:
The Biopremier Microbial DNA extraction Kit – Bead beating is designed for a fast and efficient purification of microbial DNA (gram-negative and gram-positive bacteria, yeast and fungi).
Microbial samples such as gram-positive bacteria, yeast and spores can be difficult to lyse due to their strong complex cell wall structures. The Biopremier Microbial DNA extraction Kit – Bead beating replaces enzymatic lysis by using mechanical disruption of cell wall structures with specialized beads. The Bead Microtubes can be used in combination with many compatible disruptive devices.
Beginning with a bead beating protocol, cells are lysed through a combination of mechanical force, heat and detergent. Appropriate DNA binding conditions to the Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer) to the lysate. Contaminants are removed by two efficient washing steps. Afterwards, the resulting DNA is recovered in a DNA-free Tris buffer to use for subsequent reactions
PRODUCT FEATURES:
- Bead Microtubes for efficient lysis included in combination liquid Proteinase K.
- No organic extraction or alcohol precipitation.
- Complete removal of contaminants and inhibitors for reliable downstream applications.
- Typical yield: 5 - 25 ug genomic DNA.
- Elution volume: 100 uL.
- High quality DNA obtained that can be directly used in PCR, Southern, any enzymatic reaction, cloning, etc.
- Kit available in format 50 preps format.
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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