Simultaneously detecting and differentiating Porcine Reproductive and Respiratory Syndrome Virus Type1 and 2 (PRRSV), Swine Influenza Virus (SIV) type A, Porcine Circovirus type 2 (PCV2) Antibody Test Kit
Swinecheck MP® PRRSV, SIV, PCV2 assay is a mutiplex immunoassay intended for determining the presence of antibodies to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) type 1 and 2, Swine Influenza Virus (SIV) type A, and Porcine Circovirus type 2 (PCV2) in swine serum.
SUMMARY AND EXPLANATION:
Swinecheck MP® PRRSV, SIV, PCV2 assay is a microsphere-based multiplex fluorescent immunoassay (MFIA)-type antibody detection test. A total of 4 distinct microsphere sets [coated with either PRRSV NA (type 2), PRRSV EU (type 1), SIV, and PCV2 antigens] are incubated in diluted swine serum and then washed. Successive incubations with biotinylated anti-swine IgG (Detection Antibody) and streptavidin R-phycoerythrin (SA-PE) reporter follow, each succeeded by a wash step to remove unbound reagent. Users place plates in the plate analyzer, which captures the fluorescence intensity of the microspheres and SA-PE reporter. An S/P ratio of the SA-PE on the antigen-coated microspheres above the provided cut-offs indicates antibody is present in the sample
- Multiplexed fluorometric immunoassay (MFIA)
- 5 plates
- Results available within 2.5 hours
- Tests per kit: 445 samples