Solutions commonly used as supplements in cell culture applications
Hybridoma Supplement is a chemically defined serum-free growth promoting supplement, suitable for fusion, selection and cloning of hybridoma cells. The supplement consists of ethanolamine, hydrocortisone, retinoic acid, linoleic acid, insulin and other growth factors. Since Hybridoma Supplement can be added directly to the culture medium, there I is no need for the use of feeder cells, which includes risks of overgrowth, contamination, competition for nutrients, and variations in growth factor concentrations. Hybridoma Supplement can be used with serum-free culture media and its low protein content facilitates the purification of monoclonal antibodies from the medium.