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HEPES Buffer Solution (1 M)
Item code
GTC14.0100
Technology
Others
Manufacturer
GRiSP
Variants
100mL
327
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Solutions commonly used in cell culture applications
PRODUCT:
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is widely used as a buffering agent in cell culture media. The zwitterion possesses a pKa(1) of 3 and a pKa(2) of 7.5 with useful pH ranges of 2.5-3.5 and 6.7-8.6. In comparison with bicarbonate buffer systems, HEPES is better in maintaining physiological pH despite changes in CO2 concentrations resulting from cellular activity.
HEPES is suitable for many cell culture systems as it is membrane impermeable and has limited effect on biochemical reactions.
Quantity: 100ml
Appearance: Clear colourless solution.
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