The GRS Viral DNA/RNA Purification Kit provides an efficient and fast method for the purification of high quality viral DNA and RNA from cell-free media (e.g. from serum, plasma, body fluids, and the supernatant from viral infected cell cultures). Eluted purified Nucleic Acid is suitable for all common applications, including PCR, real-time PCR, RT-PCR, One-step qRT-PCR, and DNA Sequencing. This kit is recommended for parallel purification of viral DNA (including CMV and HBV) and viral RNA (including HIV, HTLV, and HCV). The detection limit depends on the type of virus and on the sensitivity of individual PCR or RT-PCR protocols.
Principle: The GRS Viral DNA/RNA Purification Kit is optimized to eliminate the need of an internal control or carrier RNA. Lysis of DNA/RNA viruses using the lysis buffer is fast and efficient. The buffer system is optimized to allow selective binding of nucleic acids to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA/RNA is subsequently eluted with RNase-Free Water. The entire procedure can be completed within 30 minutes without the use of phenol extraction.
Sample : 200μl of cell-free medium (serum, plasma. body fluid, supernatant of viral infected cells)
Expected Yield : viral DNA and RNA (10^1-10^9 copies)
Format : spin column (certified DNase/RNase-free)
Operation Time : within 30 minutes
Elution Volume : 50μl