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GRS Total RNA Kit - Tissue
Item code
GK09.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
100 preps/ kit
322
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Total RNA isolation: for a wide variety of tissues and FFPE
PRODUCT:
The GRS Total RNA Kit – Tissue – provides an efficient and fast method for the purification and or concentration of high quality total RNA (including mRNA, tRNA and rRNA) from a variety of animal and paraffin-embedded tissues. Optional DNAse treatment can be included in the protocol to remove undesired DNA residue. Eluted purified RNA is suitable for RT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.
Principle: The GRS Total RNA Kit – Tissue – uses detergents and chaotropic salts to lyse cells and to denature proteins/inactivate RNAse. (If desired, an optional DNAse treatment step can be followed in order to remove unwanted residual DNA). The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 60 minutes with typical RNA yields of 5-30μg.
Sample : up to 25mg of tissue, up to 25 mg of paraffin-embedded tissue
Expected Yield : 5-30μg total RNA
Format : spin column (certified DNAse/RNAse-free)
Operation Time : within 60 minutes
Elution Volume : 50μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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