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GRS Total RNA Kit - Plant
Item code
GK10.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
100 preps/ kit
321
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Total RNA isolation: for plant tissue and cells
PRODUCT:
The GRS Total RNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total RNA (including mRNA, tRNA and rRNA) from plant tissue and cells. Optional DNAse treatment can be included in the protocol to remove undesired DNA residue. Eluted purified RNA is suitable for RT-PCR, One-step qRT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.
Principle: Samples are ground in liquid nitrogen and subsequently filtered to remove debris. The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 30 minutes with typical RNA yields of 5-30μg without the use of phenol extraction.
Sample : up to 100mg of fresh plant tissue, up to 25 mg of dry plant tissue
Expected Yield : 5-30μg total RNA from young leaf samples
Format : spin column (certified DNAse/RNAse-free)
Operation Time : within 30 minutes
Elution Volume : 50μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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