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GRS Total RNA Kit - Plant

GK10.0100
qPCR / Real Time PCR/ PCR
GRiSP
100 preps/ kit 

The GRS Total RNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total RNA (including mRNA, tRNA and rRNA) from plant tissue and cells. Optional DNAse treatment can be included in the protocol to remove undesired DNA residue. Eluted purified RNA is suitable for RT-PCR, One-step qRT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.Principle Samples are ground in liquid nitrogen and subsequently filtered to remove debris. The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 30 minutes with typical RNA yields of 5-30μg without the use of phenol extraction. Sample : up to 100mg of fresh plant tissue, up to 25 mg of dry plant tissue Expected Yield : 5-30μg total RNA from young leaf samples Format : spin column (certified DNAse/RNAse-free) Operation Time : within 30 minutes Elution Volume : 50μl