GRS Total RNA Kit - Blood & Cultured Cells

qPCR / Real Time PCR/ PCR
100 preps/ kit 

The GRS Total RNA Kit – Blood & Cultured Cells – provides an efficient and fast method for the purification of high quality total RNA (including mRNA, tRNA and rRNA) from fresh whole blood (human) and cultured animal cells. Eluted purified RNA is suitable for RT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.Principle The GRS Total RNA Kit – Blood & Cultured Cells – uses detergents and chaotropic salts to lyse cells and to denature proteins/inactivate RNAse. Optional DNAse treatment can be included in the protocol to remove undesired trace amounts of DNA. The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 20 minutes with typical RNA yields of 3-30μg. Sample : up to 300μl of whole fresh blood, up to 5×10^6 cultured animal cells. Expected Yield : 2-3μg total RNA (300μl blood), 25-20μg (1×10^6 293T cells) Format : spin column (certified DNAse/RNAse-free) Operation Time : within 20 minutes Elution Volume : 25-100μl