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GRS Pure DNA Kit
Item code
GK05.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
GRiSP
Variants
100 preps/ kit
312
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Genomic DNA purification: for purification and/or concentration from samples containing partial purified DNA
PRODUCT:
The GRS Pure DNA Kit provides an efficient and fast method for the purification and or concentration of high quality DNA (50bp to 30kb) from samples containing partial purified DNA (genomic DNA, mitochondrial DNA, PCR products, etc) obtained via other DNA isolation methods. The purified DNA is suitable for all common downstream applications including PCR, RFLP, cloning, library construction, Southern blot analysis, and DNA sequencing.
Principle: The GRS Pure DNA Kit uses a unique DNA Binding Buffer that is optimized to allow easy binding of DNA to the glass fiber matrix of the spin column. Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified DNA is subsequently eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in approximately 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA recovery of 80% to 90%.
Sample: up to 100μl of DNA products
Expected Yield: up to 90%
Format: spin column
Operation Time: 20 minutes
Elution Volume: 20-50 μl
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Equipments (59)
Agitators - Incubators - Vortex (9)
Autoclave Dry Heat Sterilizer (2)
Automatic Pipettors - Liquid dispensers (1)
Balances (2)
Baths - Heating thermoblock (3)
Centrifuges (4)
Electrodes pH - Conductivity - Oxygen (3)
Electrophoresis (7)
Equipments ELISA (4)
Equipments Lateral flow (3)
Equipments Real Time PCR (1)
Freezers - Refrigerators (2)
Homogenizer - Food homogenizer (3)
Microbiological incubators - Chambers (5)
Micropipettes - Electronic Pipettes (3)
Microscopes (2)
PCR - Laminar Flow Chambers (3)
Spectrophotometers (2)
Food (242)
Beverages quality (3)
Food adulteration (28)
Food allergens (67)
Food safety (45)
Genetically modified organisms (GMO) (6)
Mycotoxins (40)
Plant pathogens (27)
Water quality (19)
Other food tests (7)
Human (126)
Bacteria (51)
Covid-19 tests (16)
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Parasites (7)
Virus (42)
General laboratory solutions (173)
Antibiotics, enzymes and buffers (23)
Cloning and cell research (27)
Culture media (27)
DNA/RNA amplification and analysis (42)
DNA/RNA extraction and purification (37)
Plasticware (17)
Veterinary (378)
Bacteria (171)
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Parasites (29)
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