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GRS Plasmid Purification Kit - MINI
Item code
GK13.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
GK13.0100: 100 preps/ kit
306
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Plasmid purification: Efficient and fast method for the purification of high quality plasmid DNA from 1-6 ml of cultured bacterial cells. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning and DNA sequencing
PRODUCT:
The GRS Plasmid Purification Kit provides an efficient and fast method for the purification of highquality plasmid DNA from 1-6 ml of cultured bacterial cells. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning and DNA sequencing.
Principle: The GRS Plasmid Purification Kit is based on a modified Alkaline Lysis method used to obtain minimal genomic DNA contaminants (1). RNAse A treatment reduces contamination with RNA. Blue Lysis Buffer is added to ensure efficient cell lysis and SDS precipitation. The buffer system is optimized to allow binding of plasmid DNA in the presence of chaotropic salts to the glass fiber matrix of the spin column (2). Contaminants are removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified plasmid DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in within 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA yields of 20-30μg for high-copy number plasmids or 3-10μg for low-copy number plasmids from 4,5 ml of cultured bacterial cells.
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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