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GRS PCR & Gel Band Purification Kit
Item code
GK01.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
GK01.0100: 100 preps/ kit
298
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PCR purification/Gel extraction: Efficient and fast method for the purification and/or concentration of high quality DNA fragments (70bp to 15kb) from PCR reactions, enzymatic restriction digestion or from agarose gels. Recovers up to 95% (PCR Clean-up) or up to 90% (Gel Extraction)
PRODUCT:
The GRS PCR & Gel Band Purification Kit provides an efficient and fast method for the purification and or concentration of high quality DNA fragments (70bp to 15kb) from PCR reactions, enzymatic restriction digestion or from agarose gels. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning, library construction, Southern blot analysis, and DNA sequencing.
Principle: The GRS PCR & Gel Band Purification Kit uses chaotropic salts to dissolve agarose gel and to denature enzymes such as restriction enzymes or polymerases. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column.
Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water.
The entire procedure can be completed in approximately 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA recovery of 80% to 90% for agarose gel extraction and up to 95% for PCR or Restriction Enzyme reactions.
Sample : up to 100μl of PCR products or DNA fragments from enzymatic reactions | up to 300mg of agarose gel
Expected Yield : typical recoveries up to 95% (PCR Clean-up) or up to 90% (Gel Extraction)
Format : spin column
Operation Time : 10 minutes (PCR clean-up), 20 minutes (Gel Extraction)
Elution Volume : 20-50 μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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