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GRS microRNA Kit
Item code
GK11.0050
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
50 preps/ kit
297
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Total RNA isolation: for purification of high quality miRNAs, from fresh blood, cultured cells, tissue, and FFPE
PRODUCT:
Standard protocols for the isolation of total RNA or mRNA are not optimized for the purification of small RNA molecules, resulting in the loss of considerable amounts of micro RNAs (miRNAs) and other small cellular RNAs. Moreover, for accurate analysis of expression of miRNAs by qPCR or by microarray analysis requires the removal of the larger RNA molecules. The GRS microRNA Kit provides an efficient and fast method for the purification of high quality miRNAs (and other small cellular RNAs), with minimal contamination from large RNA molecules and genomic DNA, from a variety of samples, including fresh blood, cultured cells, tissue, and FFPE.
Principle: The GRS microRNA Kit uses chaotropic salts and various ethanol concentrations to allow selective binding of RNA of specific sizes to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with a specific RNAse-Free Elution Buffer. The entire procedure can be completed within 30 minutes.
Sample : up to 200μl of fresh blood, up to 1×10^6 cultured cells, up to 100mg of tissue, up to 25 mg of formalin-fixed paraffin-embedded tissue (FFPE)
Expected Yield : miRNA (and other small cellular RNAs)
Format : spin column (certified DNAse/RNAse-free)
Operation Time : 30 minutes
Elution Volume : 50μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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ELISA
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Lateral Flow Test
MFIA
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