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GRS microRNA Kit

GK11.0050
qPCR / Real Time PCR/ PCR
GRiSP
50 preps/ kit 

Standard protocols for the isolation of total RNA or mRNA are not optimized for the purification of small RNA molecules, resulting in the loss of considerable amounts of micro RNAs (miRNAs) and other small cellular RNAs. Moreover, for accurate analysis of expression of miRNAs by qPCR or by microarray analysis requires the removal of the larger RNA molecules. The GRS microRNA Kit provides an efficient and fast method for the purification of high quality miRNAs (and other small cellular RNAs), with minimal contamination from large RNA molecules and genomic DNA, from a variety of samples, including fresh blood, cultured cells, tissue, and FFPE.Principle The GRS microRNA Kit uses chaotropic salts and various ethanol concentrations to allow selective binding of RNA of specific sizes to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with a specific RNAse-Free Elution Buffer. The entire procedure can be completed within 30 minutes. Sample : up to 200μl of fresh blood, up to 1×10^6 cultured cells, up to 100mg of tissue, up to 25 mg of formalin-fixed paraffin-embedded tissue (FFPE) Expected Yield : miRNA (and other small cellular RNAs) Format : spin column (certified DNAse/RNAse-free) Operation Time : 30 minutes Elution Volume : 50μl