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GRS Genomic DNA Kit - Plant
Item code
GK04.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
100 preps/ kit
287
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Genomic DNA purification: for Plant tissue and cells
PRODUCT:
The GRS Genomic DNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA, and chloroplast DNA) from plant tissue and cells. Eluted purified DNA is suitable and ready-to-use for PCR, real-time PCR, Southern Blotting and RFLP.
Principle: Samples are ground in liquid nitrogen and further disrupted by incubation in lysis buffer. The lysate is treated with RNAse A to degrade RNA and subsequently filtered to remove cell debris and other precipitates. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified DNA is subsequently eluted with a low salt Elution Buffer (or TE). The entire procedure can be completed within 60 minutes without phenol extraction or ethanol precipitation with typical DNA yields of 5-20μg (up to 50μg).
Sample : up to 100mg of fresh plant tissue, up to 25 mg of dry plant tissue
Expected Yield : 3-5μg total DNA from 100mg Arabidopsis thaliana young leaf samples 20-25μg total DNA from 100mg Nicotiana tabacum young leaf samples
Format : spin column
Operation Time : within 60 minutes
Elution Volume : 50-200μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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