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GRS Genomic DNA Kit - Blood & Cultured Cells
Item code
GK02.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
100 preps/ kit
283
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Genomic DNA purification: for whole and frozen blood, buffy coat and cultured animal cells (available adaptation, for bacteria and fungus)
PRODUCT:
The GRS Genomic DNA Kit – Blood & Cultured Cells – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA and viral DNA) from a variety of samples, including whole and frozen blood, buffy coat and cultured animal cells. Eluted purified DNA (approximately 20-30kb) is suitable for PCR, and other enzymatic reactions.
Principle: The GRS Genomic DNA Kit – Blood & Cultured Cells – uses chaotropic salts to lyse cells and to denature proteins. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column.
Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step.
The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed within an hour without phenol/chloroform extraction or alcohol precipitation, with typical DNA yields of 6μg from 200μl of whole human blood or up to 50μg from 200μl of buffy coat.
Sample : up to 300μl of whole blood, up to 200μl of frozen blood, up to 200μl of buffy coat, up to 1×10^7 cultured animal cells.
Expected Yield : up to 50μg DNA
Format : spin column
Operation Time: within 60 minutes
Elution Volume: 50-200 μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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