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GRS Genomic DNA Kit - Bacteria
Item code
GK07.0100
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Research Solutions
Variants
100 preps/ kit
282
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Genomic DNA purification: for Gram (+) positive and Gram (-) negative bacteria
PRODUCT:
The GRS Genomic DNA Kit – Bacteria – provides an efficient and fast method for the purification of high quality genomic (and viral) DNA from Gram-positive and Gram-negative bacteria, suitable for all common downstream applications such as PCR, enzymatic restriction digestion, cloning, Southern blot analysis, etc.
Principle: The GRS Genomic DNA Kit – Bacteria – can be used for both Gram (-) and Gram (+) bacterial cells. The provided Buffer G+, once supplemented with lysozyme, will lyse bacterial cell walls consisting of peptidoglycan. Chaotropic salt is then used for further cell lysis and protein degradation. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column. Contaminants such as proteins, divalent cations, secondary metabolites, and enzyme inhibitors are completely removed using Wash Buffer.
The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in less than 60 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA yield of 25-30 μg from 1, 5ml of Escherichia coli (~10^9 cells).
Sample : 0,5-2,0 ml of bacterial culture with up to 1×10^9 cells (Gram (+) or Gram (-))
Expected Yield : 25-30 μg (1×10^9 of Escherichia coli); 10-15 μg (1×10^9 of Bacillus subtilis)
Format : spin column
Operation Time : less than 1 hour
Elution Volume : 30-200 μl
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Filters
Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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