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Exonuclease I (20U/ul)
Item code
GE014.0001
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Research Solutions
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1mL (20.000U)
253
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Ribonuclease A is a pancreatic endoribunuclease that specifically cleaves single-stranded RNA at the 3’ end of pyrimidine residues. Lyophilized powder purified from bovine pancreas.
PRODUCT:
Exonuclease I (Exo I) is an exonuclease that hydrolyzes single-stranded DNA, one nucleotide at a time from the end, in the 3´→5´ direction. It releases 5´- mononucleotides one after another and leaves the terminal 5´-dinucleotide intact. This 55kDa enzyme, product of the E.coli sbcB gene, does not cleave DNA strands without terminal 3´-hydroxyl groups, as these are blocked by phosphoryl or acetyl groups1. It does not degrade double-stranded DNA. For activity, Exo I requires magnesium (optimal [Mg2+] is 10mM). Exo I is tolerant to a wide variety of buffer conditions (salt, pH, etc), and thus can be added directly to most molecular biology buffers containing magnesium (>1mM), including PCR reaction mixtures.
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
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Bacteria
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Virus
Other microrganisms
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