GO
COOKIES - This website uses its own and third party cookies to help provide you with the best experience we can. By clicking “I Accept" or scrolling down this page you accept cookies.
I Accept Read Full Policy

CAT_MenuNF

Menu
Filters
_CAT_SearchNF_mobile

Search

Exonuclease I (20U/ul)

GE014.0001
Others
Research Solutions
1mL (20.000U)
Add To Wish List
Request quote
Exonuclease I (20U/ul)

Ribonuclease A is a pancreatic endoribunuclease that specifically cleaves single-stranded RNA at the 3’ end of pyrimidine residues. Lyophilized powder purified from bovine pancreas.

PRODUCT: Exonuclease I (Exo I) is an exonuclease that hydrolyzes single-stranded DNA, one nucleotide at a time from the end, in the 3´→5´ direction. It releases 5´- mononucleotides one after another and leaves the terminal 5´-dinucleotide intact. This 55kDa enzyme, product of the E.coli sbcB gene, does not cleave DNA strands without terminal 3´-hydroxyl groups, as these are blocked by phosphoryl or acetyl groups1. It does not degrade double-stranded DNA. For activity, Exo I requires magnesium (optimal [Mg2+] is 10mM). Exo I is tolerant to a wide variety of buffer conditions (salt, pH, etc), and thus can be added directly to most molecular biology buffers containing magnesium (>1mM), including PCR reaction mixtures.

Newsletter

×