Immunoenzymatic assay for the detection of antibodies against transmissible gastroenteritis virus (TGEV) in porcine serum.
This kit is based on an immunoenzymatic assay for the detection of antibodies against transmissible gastroenteritis virus (TGEV) in porcine serum
SUMMARY AND EXPLANATION:
Transmissible gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by TGEV. TGEV affects swine of all ages. It multiplies in the digestive and respiratory tracts and causes vomiting and diarrhea. The mortality rate in piglets under 2 weeks of age is near 100%. In older pigs, the disease is milder and can even
occasionally go unnoticed.
A fast and accurate diagnosis of TGE is essential to prevent dissemination of the disease. Diagnosis is mostly based on viral identification. However detection of antibodies is very useful in older pigs that do not always display typical signs of disease. Serological tests are also regularly required for commercial exchanges.
Antibodies against TGEV can be detected using the virus neutralization assay (VN). However, the VN test requires cell culture facilities and the use of infectious TGEV which limits its use to specialized laboratories.
Moreover, it is very time consuming and results are available after several days only. By contrast, enzyme-linked immunosorbent assays (ELISA) can be run in most laboratories and results are available within a few hours.
Use of monoclonal antibodies specific to TGEV in a blocking ELISA assay allows the detection of TGEV antibodies whereas antibodies directed against porcine respiratory coronavirus (PRCV), a closely related swine coronavirus, are not detected. The specificity of the assay is improved by using a recombinant protein
- Blocking ELISA: Specific antigens to TGEV
- Rapid (less than 2 hours)
- Test per kit: 460