Immunoenzymatic assay for the detection and differentiation of antibodies against transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in porcine serum.
This kit is based on an immunoenzymatic assay for the detection and differentiation of antibodies against transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in porcine serum
SUMMARY AND EXPLANATION:
Transmissible gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by TGEV. TGEV affects swine of all ages. It multiplies in the digestive and respiratory tracts and causes vomiting and diarrhea. The mortality rate in piglets under 2 weeks of age is near 100%. In older pigs, the disease is milder and can even occasionally go unnoticed.
PRCV is a respiratory variant of TGEV which has lost the ability to colonize the digestive tract. PRCV infects swine of all ages and can cause benign respiratory infections. Its close relationship with TGEV complicates the diagnosis of TGE.
A fast and accurate diagnosis of TGE is essential to prevent dissemination of the disease. Diagnosis is mostly based on viral identification. However detection of antibodies is very useful in older pigs that do not always display typical signs of disease. Serological tests are also regularly required for commercial exchanges.
As antibodies against TGEV and PRCV show complete cross-reaction by neutralization, the differentiation of antibodies aimed against these two viruses is impossible by seroneutralization testing. However, the use of monoclonal antibodies in a competitive ELISA assay allows the detection and differentiation of infections caused by these two viruses. The specificity of the assay is improved by using a recombinant protein as antigen.
- Blocking ELISA: Specific antigens to TGEV
- Rapid (less than 2 hours)
- Test per kit: 88 samples