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DNA Standard SUPREME Alaska pollack Codfish (Gadus chalcogrammus)

BIOPDNA-058
qPCR / Real Time PCR/ PCR
Biopremier
700 uL
(pdf) Instructions for use
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DNA Standard SUPREME Alaska pollack Codfish (Gadus chalcogrammus)

DNA relative quantification in combination with the SUPREME Real Time Detection Kit

BACKGROUND: According to the European Commission Directive 2002/86/EC, food ingredients must be declared. Additionally, according to the EU Directive 1379/2013, the fish species must be disclosed on the label with both commercial and scientific denominations in order to verify the origin and traceability of the fish and to analyze the quality control of handling and cleaning processes of production lines.
Moreover, the species authenticity is also highly relevant to the consumer for diverse reasons such as economic, health, cultural and religious. Fraudulent substitution of cheaper meats/fishes in place of more expensive ones, the inclusion of meat in non-meat/fish (vegetarian) products and the interest of knowing the animal speciation in meat products (halal and kosher) are clear examples for the importance of this issue.
Several methods for species detection are based on the identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, DNA methodologies like PCR based techniques still allow the identification of DNA from the dierent species present in a sample. Real time PCR techniques, using specific fluorescent probes, are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity.

PRODUCT DESCRIPTION: DNA Standard SUPREME Alaska pollack (Gadus chalcogrammus) is designed to perform a comparative quantitation analysis of Alaska pollack (Gadus chalcogrammus) DNA in food and feedstuff samples by Real Time PCR. For reliable quantitative results it is recommended to apply the DNA Standard SUPREME Alaska pollack (Gadus chalcogrammus) in combination with the SUPREME Real Time Detection Kit Alaska pollack (Gadus chalcogrammus) (BIOPSFS-058 or BIOPSFS-sp058)

PRODUCT FEATURES:
- Contain a fragment of DNA at a known concentration to be used as a standard
-SUPREME Real Time Detection Kit Alaska pollack (Gadus chalcogrammus) (BIOPSFS-058 or BIOPSFS-sp058) (not included but necessary)

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