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Blood DNA Extraction Kit Biopremier

BIOPEXT-0606.250
qPCR / Real Time PCR/ PCR
Biopremier
BIOPEXT-0606.250: 250 preps
BIOPEXT-0606.50: 50 preps

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Blood DNA Extraction Kit Biopremier

The Biopremier BLOOD DNA extraction Kit is designed for the rapid purification of pure genomic DNA from whole blood, serum, plasma, body fluids and dried blood spots.

PRODUCT DESCRIPTION: The Biopremier BLOOD DNA extraction Kit is designed for the rapid purification of pure genomic DNA from whole blood, serum, plasma, body fluids and dried blood spots.
The procedure involves the processing of either whole blood or DNA isolated from leukocytes, preceded by the lysis of erythrocytes using the RBC lysis buffer provided in the kit. Then, lysis is completed by incubation of whole blood in a solution containing chaotropic ions in presence of proteinase K at 70ºC. Appropriate conditions for binding DNA to the silica membrane are created by addition of ethanol to the lysate. Contaminants are removed by efficient washing with two different buffers. Pure genomic DNA is finally eluted under low ionic strength in a slightly alkaline elution buffer and it’s ready to use for subsequent reactions.

PRODUCT FEATURES:
- No organic extraction or alcohol precipitation.
- Complete removal of contaminants and inhibitors for reliable downstream applications.
- Regular yield: 4- 6 ug genomic DNA.
- Elution volume: 50-200 uL.
- High quality DNA obtained that can be directly used in PCR and other aplications such as Southern, any enzymatic reactions or cloning.
- Compatible with BIOPREMIER Real time detetion kits and others Real Time detection / End-Point Kits
- Available formats: Kits with 250 or 50 preps

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