BactoReal® Kit Mycobacterium tuberculosis complex (MTBC)

Detection of the ITS2 region of the MTBC (Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium canetti, Mycobacterium africanum and Mycobacterium microti)

Background: Members of the Mycobacterium tuberculosis-complex (MTC) cause tuberculosis in animals and humans. The eight closely related species of the MTC have a wide range of natural hosts including bovine hosts (M. bovis), caprine hosts (M. caprae), rodent hosts (M. microti), and pinniped hosts (M. pinnipedii). Bovine tuberculosis remains an important disease in many countries of the world, causing significant economic losses. Infected cattle can excrete the causative agent of tuberculosis via the respiratory tract, faeces or milk depending on the affected organ system.

Description: BactoReal® Kit Mycobacterium tuberculosis complex uses real-time PCR for the ITS2 region of Mycobacterium species belonging to the Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. caprae, M. canetti, M. africanum and M. microti). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: ITS2 region of Mycobacterium tuberculosis complex
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX