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BactoReal® Kit Histophilus somni
Item code
DVEB06111
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Ingenetix
Variants
DVEB06111: 100 reactions - FAM, VIC/HEX
DVEB06113: 100 reactions - FAM, Cy5
DVEB06151: 50 reactions - FAM, VIC/HEX
DVEB06153: 50 reactions - FAM, Cy5
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Detection of the 16S rRNA gene of Histophilus somni
Background:
Histophilus somni is an unencapsulated gram-negative bacterium and a member of the Pasteurellaceae family. It is a common commensal in the lower reproductive and upper respiratory tract of cattle and other ruminants. H. somni is one of the bacterial agents responsible for bovine respiratory disease (BRD) complex and associated with respiratory disease, arthritis, mastitis, myocarditis and reproductive failure in sheep and cattle. Other important bacterial pathogens of BRD are Pasteurella multocida, Mannheimia haemolytica, Trueperella pyogenes and Mycoplasma bovis. Mixed infections with these organisms do occur.
Description:
BactoReal® Kit Histophilus somni uses real-time PCR for the detection of the 16S rRNA gene of Histophilus somni. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Product Features:
- Amplification and detection: 16S rRNA gene of Histophilus somni
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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Technology
ELISA
qPCR / Real Time PCR/ PCR
Others
Lateral Flow Test
MFIA
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