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BactoReal® Kit E. coli Typing (STEC) stx1 & stx2
Item code
DVEB04013
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Ingenetix
Variants
DVEB04013: 100 reactions - FAM, VIC/HEX, Cy5
DVEB04053: 50 reactions - FAM, VIC/HEX, Cy5
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Detection of virulence-associated genes stx1 and stx2 (verotoxin) of Escherichia coli
Background:
Escherichia coli is a Gramnegative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors, which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. Enteropathogenic Escherichia coli (EPEC) produces an outer membrane protein called intimin, encoded by eae and mediating intestinal cell attachment. Intimin is a virulence factor (adhesin) of EPEC and EHEC strains. Furthermore, EPEC are classified into typical and atypical strains based on the presence of the plasmid E. coli adherence factor (EAF). Typical EPEC strains are rarely isolated from animals, and humans are the major natural reservoir. The reservoir for atypical EPEC can be both animals and humans. By definition all EPEC lack genes to produce shiga toxin (stx).
Description:
BactoReal® Kit E. coli Typing is a series of different real-time PCR kits for detection of virulence-associated genes of E. coli. Depending on which kit and which combinations of kits are used, they allow the typing of enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and of Shiga toxin (verotoxin) producing E. coli (STEC). Assays for stx1 and stx2, as well as assays for eae and ehxA can be combined in a multiplex PCR. All kits consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Product Features:
- Amplification and detection: virulence-associated genes stx1 & stx2 of E. coli
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
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Virus
Other microrganisms
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