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Aflatoxin B1 ELISA
Item code
DEAB1E03
Technology
ELISA
Manufacturer
Diagnostics
Variants
96 tests
669
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Enzyme immunoassay for the rapid quantitative determination of Aflatoxin B1 in cereals and beer/gyle.
Background:
Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocumarines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed. Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions.
In the European Union the limits for aflatoxin B1 are 2 – 12 ppb for regular food products. Thus a monitoring of food and feed with respect to the concentration of aflatoxin B1 is obligatory. Aflatoxin B1 ELISA represents a highly sensitive detection system and is particularly capable of the rapid quantification of aflatoxin B1 contaminations in cereals and beer.
Description:
The Aflatoxin B1 quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a microtiter plate. Aflatoxin B1 containing samples or standards, an aflatoxin-peroxidase conjugate and an antibody directed against aflatoxins are given into the wells of the microtiter plate. The conjugate competes with the aflatoxin B1 of samples/standards for the limited number of antibody sites. Simultaneously the anti-aflatoxin antibody is bound to the antibody-binding protein coated on the microtiter plate. After 10 minutes incubation at room temperature the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 10 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of aflatoxin B1 is indirectly proportional to the colour intensity of the test sample.
Product Features:
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Aflatoxin B1 Standards: 0, 1.5, 3, 6, 12, 24 ppb;
- ELISA reader at 450 nm;
- Standard range: 1.5 - 24 ppb;
- Sensitivity analytical: 0.5 ppb.
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
Antibiotics, enzymes and buffers
Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
Fungi
Hormones
Parasites
Virus
Other microrganisms
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MFIA
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