Vasoactive Intestinal Polypeptide (VIP) ELISA

Enzyme immunoassays for the quantitative measurement of Vasoactive Intestinal Polypeptide (VIP) in human EDTA plasma.

INTENDED USE:For the quantitative measurement of Vasoactive Intestinal Polypeptide (VIP) in human EDTA plasma by an ELISA (Enzyme-Linked Immunosorbent Assay).

GENERAL INFORMATION:The VIP ELISA is a two-step competitive immunoassay. In the first incubation step, competition occurs between VIP present in calibrators, controls, specimen samples and a biotin-labelled antigen (biotin conjugate) for a limited number of anti-VIP antibody binding sites on the microplate wells. Excess and unbound materials are removed by a washing step. In the second incubation step, streptavidin-HRP conjugate is added, which binds specifically to any bound biotin conjugate. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of VIP present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm.A set of calibrators is used to plot a calibrator curve from which the amount of VIP in specimen samples and controls can be directly read.

KIT CHARACTERISTICS:
- Method: ELISA br>- Tests: 96 br>- Incubation Time / Conditions: 30 min, 1 h, 30 min, 30 min (RT/shaker) br>- Standard Range: 25 - 800 pg/ml br>- Final Sample Volume: 75 µl br>- Sample Type: plasma (EDTA) br>- Isotope / Substrate: TMB 450 nm br>- Internal Controls: 2 br>- Regulatory Status: RUO