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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | BIOPEXT-0605.250: 250 preps BIOPEXT-0605.50: 50 preps |
| Technical Sheet | Download PDF |
Description
The Biopremier TISSUE Genomic DNA extraction Kit is designed for the rapid purification of highly pure genomic DNA from a wide variety of samples.
PRODUCT DESCRIPTION: The Biopremier TISSUE Genomic DNA extraction Kit is designed for the rapid purification of highly pure genomic DNA from a wide variety of samples, including whole blood, culture cells, animal tissues, mouse tails, bacteria, yeats, clinic samples (serum, plasma, feces, urine), forensic samples and paraffin-embedded tissues.
Cell lysis is achieved by incubation of the sample in a solution containing SDS and proteinase K at 55ºC. Appropriate conditions for binding DNA to the glass fiber membrane are created by addition of chaotropic ions to the lysate. Contaminants are removed by efficient washing with wash buffer. Pure genomic DNA is finally eluted with an elution buffer and it’s ready to use for subsequent reactions.
PRODUCT FEATURES:
- No organic extraction or alcohol precipitation.
- Complete removal of contaminants and inhibitors for reliable downstream applications.
- Regular yield: 25- 35 ug genomic DNA.
- Binding capacity: 60 ug.
- Elution volume: 50-200 uL.
- High quality DNA obtained that can be directly used in PCR or other applications such as Southern, any enzymatic reactions or cloning
- Compatible with BIOPREMIER Real time detetion kits and others Real Time detection / End-Point Kits
-Available formats: Kits with 250 or 50 preps
PRODUCT DESCRIPTION: The Biopremier TISSUE Genomic DNA extraction Kit is designed for the rapid purification of highly pure genomic DNA from a wide variety of samples, including whole blood, culture cells, animal tissues, mouse tails, bacteria, yeats, clinic samples (serum, plasma, feces, urine), forensic samples and paraffin-embedded tissues.
Cell lysis is achieved by incubation of the sample in a solution containing SDS and proteinase K at 55ºC. Appropriate conditions for binding DNA to the glass fiber membrane are created by addition of chaotropic ions to the lysate. Contaminants are removed by efficient washing with wash buffer. Pure genomic DNA is finally eluted with an elution buffer and it’s ready to use for subsequent reactions.
PRODUCT FEATURES:
- No organic extraction or alcohol precipitation.
- Complete removal of contaminants and inhibitors for reliable downstream applications.
- Regular yield: 25- 35 ug genomic DNA.
- Binding capacity: 60 ug.
- Elution volume: 50-200 uL.
- High quality DNA obtained that can be directly used in PCR or other applications such as Southern, any enzymatic reactions or cloning
- Compatible with BIOPREMIER Real time detetion kits and others Real Time detection / End-Point Kits
-Available formats: Kits with 250 or 50 preps
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