Detection of animal DNA in food products and animal feedstuff. For use in real time instruments with FAM, ROX detection channels.
BACKGROUND: Species authenticity is highly relevant to consumers for economic, medical, cultural, and religious reasons. Examples like fraudulent substitution of expensive species with cheaper meats, inclusion of meat in vegetarian products, and allergens in food products highlight the importance of this issue.
With a growing number of individuals adopting vegetarian or vegan lifestyles, it becomes essential to certify the absence of animal-derived ingredients in vegan food to maintain consumer confidence in the supply chain and label claims.
Products that advertise being vegan must be checked for this during or after production by food production laboratories or control authorities to protect the consumer from fraud or mislabeling. The, e.g., European V-label defines in its guidelines for unintentional traces of animal residues a limit of less than 0.1% (1 g/kg) of animal ingredients.
The BIOPREMIER SUPREME REAL TIME DETECTION KIT VEGAN is specifically designed to detect animal traces in food and feed samples using qPCR, addressing the need for reliable detection methods in the face of global food fraud risks. Vegan food manufacturers and suppliers can employ this kit to validate the authenticity of their products and ensure compliance with vegan standards. It allows them to detect even trace amounts of animal DNA in food samples, including raw ingredients, processed foods, and finished products.
Traditional protein-based detection methods are unreliable for highly processed and heated products, unlike DNA-based methods. DNA is more stable, and modern PCR techniques enable the identification of fragmented DNA from the species present in a sample, ensuring accurate and highly sensitive detection with a lower detection limit detection.
PRODUCT DESCRIPTION: SUPREME REAL TIME DETECTION VEGAN uses real-time PCR for the detection of meat DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target as well as a positive control and an internal control (IC).
PRODUCT FEATURES:
-Amplification and detection of animal DNA
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other SUPREME BIOPREMIER kits simultaneously
-Channel Target: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions